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乙酰胺肉汤
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更新时间:2016-06-11  |  阅读:1168

详情介绍

乙酰胺肉汤 Acetamide broth 250g 用于铜绿假单菌产氨试验。

用途:用于铜绿假单菌产氨试验

乙酰胺肉汤 配方:(g/L)
磷酸二氢钾
氯化钠
硫酸镁
乙酰胺
钼酸钠
硫酸亚铁
pH值7.1-7.3   
1.0
0.2
0.2
2.0
0.005
0.0005
25℃
原理:
       具有乙酰胺酶的细菌能利用乙酰胺作为碳源,并分解乙酰胺产碱;氯化钠维持均衡的渗透压;磷酸二氢钾为缓冲剂;硫酸镁提供生长必要的离子。
用法:
       称取本品  3.4g, 加热搅拌溶解于1000ml蒸馏水中,分装到试管中,每管 5ml,121℃高压灭菌15min ,备用。
  注:本培养基高压灭菌后有沉淀物
质量控制:

 在36±1℃培养18-24小时。

质控菌株
结果
铜绿假单胞菌
+(砖红色)
大肠埃希氏菌
-(淡)

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