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脑-心浸萃琼脂培养基
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更新时间:2016-06-10  |  阅读:1248

详情介绍

脑-心浸萃琼脂培养基 Brain-extracted agar Baptist Heart 250g 用于粪链球菌的纯化培养。

用途:用于粪链球菌的纯化培养

脑-心浸萃琼脂培养基配方:(g/L)
牛脑浸粉
牛心浸粉
蛋白胨
酪蛋白胨
氯化钠
葡萄糖
磷酸氢二钠
琼脂
pH值7.4 ± 0.2 
4.0
4.0
5.0
16.0
5.0
2.0
2.5
13.5
25℃
原理:
       牛脑浸粉、牛心浸粉、蛋白胨、酪蛋白胨提供氮源、维生素和生长因子;葡萄糖可为多种细菌提供能源;氯化钠维持均衡的渗透压;磷酸氢二钠为缓冲剂。
用法:
          称取本品 52.0g,加热搅拌溶解于 1000ml 蒸馏水中,121℃高压灭菌 15 分钟,备用。
质量控制:

质控菌株接种后于35±0.5℃培养24h,结果如下:

菌    名 
菌    号
生长状况 
粪性链球菌
CMCC(B)32223 
良好
乙型溶血性链球菌
CMCC(B)32210
良好
大肠埃希氏菌 
ATCC25922 
良好

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