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明胶培养基基础
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更新时间:2016-06-13  |  阅读:2513

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明胶培养基基础 Gelatin Medium Base 250g 生化试验培养基,用于细菌明胶液化试验(GB标准)

用途:生化试验培养基,用于细菌明胶液化试验(GB标准)

明胶培养基基础配方:(g/L)
牛肉粉
蛋白胨
明胶
pH值7.1 ± 0.1 
3.0
5.0 
120.0
25℃
原理:
        蛋白胨和牛肉粉提供氮源、维生素、矿物质;某些含明胶酶的细菌水解明胶,使其液化而降低明胶的胶凝作用。
用法:
        称取本品 128.0g,加热溶解于1000ml蒸馏水中,121℃ 高压灭菌 20 分钟,备用。
质量控制:

 在37培养18-24小时。

质控菌株
ATCC
生长情况
液化情况
金葡萄球菌
25923
+/-
-
产气荚膜梭菌
 
+++
+

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