详情介绍
双料乳糖胆盐(含中和剂)培养基 | Double Lactose Bile Medium | 250g | 用于化妆品中粪大肠菌群的测定(GB标准) |
用途:用于化妆品中粪大肠菌群的测定(GB标准)
双料乳糖胆盐(含中和剂)培养基配方:(g/L) | |||||||||||||||||||||||||||||
蛋白胨 乳糖 牛胆盐 溴甲酚紫 pH值 7.4 ± 0.2 | 40.0 10.0 20.0 0.02 25℃ | ||||||||||||||||||||||||||||
原理: | |||||||||||||||||||||||||||||
蛋白胨提供氮源,维生素,生长因子;乳糖提供能源;牛胆盐抑制革兰氏阴性菌生长;溴甲酚紫作为指示剂。 | |||||||||||||||||||||||||||||
用法: | |||||||||||||||||||||||||||||
称取本品 70.0g,溶解于1000ml 蒸馏水中,分装到有倒立发酵管的20mm×150mm试管中,每管10ml,115℃高压灭菌 15 分钟,备用。 | |||||||||||||||||||||||||||||
质量控制: | |||||||||||||||||||||||||||||
在36±1℃培养18-24小时。
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