详情介绍
乳糖胆盐培养基 | Lactose Bile Medium | 250g | 用于化妆品中粪大肠菌群的测定(GB标准) |
用途:用于化妆品中粪大肠菌群的测定(GB标准)
乳糖胆盐培养基配方:(g/L) | |||||||||||||||||
蛋白胨 乳糖 牛胆盐 溴甲酚紫 pH值 7.4 ± 0.1 | 20.0 5.0 5.0 0.01 25℃ | ||||||||||||||||
原理: | |||||||||||||||||
蛋白胨提供碳源和氮源满足细菌生长的需求;猪胆盐可抑制革兰氏阳性细菌的生长;乳糖是大肠菌群可发酵的糖类;溴甲酚紫是pH指示剂,酸性呈,碱性呈紫色。 | |||||||||||||||||
用法: | |||||||||||||||||
称取本品 30.0g,加热溶解于 1000ml 蒸馏水中,分装, 115℃高压灭菌 20 分钟,备用。 | |||||||||||||||||
质量控制: | |||||||||||||||||
在44±0.5培养18-24小时。
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