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乳糖胆盐培养基
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更新时间:2016-06-14  |  阅读:1340

详情介绍

乳糖胆盐培养基 Lactose Bile Medium 250g 用于化妆品中粪大肠菌群的测定(GB标准)

用途:用于化妆品中粪大肠菌群的测定(GB标准)

乳糖胆盐培养基配方:(g/L)
蛋白胨
乳糖
牛胆盐
溴甲酚紫
pH值 7.4 ± 0.1  
20.0
5.0
5.0
0.01
25℃
原理:
         蛋白胨提供碳源和氮源满足细菌生长的需求;猪胆盐可抑制革兰氏阳性细菌的生长;乳糖是大肠菌群可发酵的糖类;溴甲酚紫是pH指示剂,酸性呈,碱性呈紫色。 
用法:
         称取本品 30.0g,加热溶解于 1000ml 蒸馏水中,分装, 115℃高压灭菌 20 分钟,备用。
质量控制:

 在44±0.5培养18-24小时。

质控菌株
菌株编号
生长情况
其它特征
金葡萄球菌
ATCC25923
-
-
大肠埃希氏菌
ATCC25922
+++
产气、变黄
沙门氏菌
ATCC14028
+++
不产气、不变黄

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