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乳糖发酵培养基
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更新时间:2016-06-13  |  阅读:2645

详情介绍

乳糖发酵培养基 Lactose Broth 250g 用于大肠菌群发酵试验。

用途:用于大肠菌群发酵试验

乳糖发酵培养基配方:(g/L)
蛋白胨
乳糖
溴甲酚紫
pH值7.4 ± 0.1        
20.0 
10.0 
0.01
25℃
原理:
        蛋白胨提供碳源和氮源满足细菌生长的需求;乳糖是大肠菌群可发酵的糖类;溴甲酚紫是pH指示剂,酸性呈,碱性呈紫色。 
用法:
         称取本品  30.0g,加热溶解于1000ml 蒸馏水中,分装到有倒立发酵管的 20mm ×150mm 
试管中,每管 10ml, 115℃高压灭菌15 分钟,备用。
质量控制:

 在36±1℃培养18-24小时。

质控菌株
菌种号
生长情况
产气情况
大肠杆菌
ATCC 25922
(10-8)+++
+
大肠菌群
 
(10-8)+++
+
金葡萄球菌
ATCC 25923
-

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