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麦康凯琼脂培养基
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更新时间:2016-06-11  |  阅读:1665

详情介绍

麦康凯琼脂培养基 MacConkey Agar 250g 用于肠道致病菌的选择性分离培养。

用途:用于肠道致病菌的选择性分离培养

麦康凯琼脂培养基配方:(g/L)
蛋白胨
乳糖
牛胆盐
氯化钠
中性红
琼脂
PH值
20.0g
10.0g
5.0g
5.0g
0.03g  
14.0g
7.1±0.2
原理:
          蛋白胨提供碳氮源、维生素和生长因子;牛胆盐可抑制革兰氏阳性菌的生长;氯化钠维持均衡的渗透压;琼脂是培养基的凝固剂;乳糖为可发酵的糖类;中性红是pH指示剂,细菌发酵乳糖产酸时菌落呈粉红色并在菌落周围出现胆盐沉淀的浑浊圈。   
用法:
     称取本品54克,加热溶解于 1000ml蒸馏水中,121℃高压灭菌15分钟备用。
质量控制:

 在36±1℃培养18-24小时。

质控菌株
菌株编号
生长情况
其它特征
金葡萄球菌
ATCC25923
-
 /
大肠埃希氏菌
ATCC25922
+++
红色菌落、有胆盐沉淀环
沙门氏菌
ATCC14028
+++
无色大菌落

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