详情介绍
4-甲基伞形酮葡糖苷酸(MUG)培养基 | MUG Medium | 100g | 用于大肠杆菌酶底物法检测。 |
用途:用于大肠杆菌酶底物法检测
4-甲基伞形酮葡糖苷酸(MUG)培养基 配方:(g/L) | |||||||||||||||||||||||||
蛋白胨 磷酸二氢钾 磷酸氢二钠 硫酸锰 亚硫酸钠 去氧胆酸钠 硫酸镁 氯化钠 氯化钙 MUG 硫酸锌 pH值7.3 ± 0.1 | 10.0 0.9 6.2 0.0005 0.04 1.0 0.1 5.0 0.05 0.075 0.0005 25℃ | ||||||||||||||||||||||||
原理: | |||||||||||||||||||||||||
蛋白胨提供碳氮源;硫酸锰、硫酸锌、硫酸镁、氯化钠和氯化钙提供各种离子可维持均衡的渗透压及利于目标菌的生长;磷酸二氢钾和磷酸氢二钠为缓冲剂;亚硫酸钠和去氧胆酸钠为选择性抑菌剂。 细菌的葡萄糖醛酸苷酶在碱性条件下,作用于4-甲基伞形酮-β-D葡萄糖醛酸苷(4-Methylumbellifery-β-D-Glucuronide简称MUG)的β糖醛酸苷键,使其水解,释放的4-甲基伞形酮在366nm紫外灯下产生蓝白色荧光。97%的大肠埃希氏杆菌、10%的沙门氏菌以及少量的志贺氏菌具有葡萄糖醛酸苷酶。 | |||||||||||||||||||||||||
用法: | |||||||||||||||||||||||||
称取本品 23.37g,加热搅拌溶解于 1000ml 蒸馏水中,分装试管,每管 5ml,115℃高压灭菌 20 分钟,备用。 注:本培养基有少许沉淀。 | |||||||||||||||||||||||||
质量控制: | |||||||||||||||||||||||||
在36±1℃培养18-24小时。
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