详情介绍
支原体肉汤培养基 | Mycoplasma Broth Medium | 250g | 用于支原体的增菌培养。 |
用途:用于培养支原体的基础培养基
支原体肉汤培养基配方:(g/L) | |||||||||||||||||
猪胃消化物 牛肉浸粉 酵母浸粉 氯化钠 葡萄糖 酚红 pH值7.6 ± 0.2 | 10.0 5.0 5.0 2.5 5.0 0.02 25℃ | ||||||||||||||||
原理: | |||||||||||||||||
猪胃消化物、牛肉浸粉、酵母浸粉提供氮源、维生素和矿物质,氯化钠维持均衡的渗透压,葡萄糖提供碳源,酚红是指示剂。 | |||||||||||||||||
用法: | |||||||||||||||||
称取本品 27.52g,加热溶解于 800ml 蒸馏水中,121℃ 高压灭菌 15 分钟,冷至室温,无菌操作加入 灭活小牛血清或马血清 200ml 和青霉素 80 万单位,混匀,分装无菌试管,备用。 | |||||||||||||||||
质量控制: | |||||||||||||||||
在36±1℃培养7天。
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