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血琼脂基础2号
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更新时间:2016-06-08  |  阅读:1502

详情介绍

血琼脂基础2号 Blood Agar Base N0.2 250g 供分离营养要求非常高的致病菌用,后加血液制成血平板。

用途:供分离营养要求非常高的致病菌用,后加血液制成血平板

血琼脂基础2号配方:(g/L)
特殊蛋白胨
氯化钠
琼脂
pH值7.4 ± 0.2 
23.0
5.0  
12.0
25℃
原理:
         特殊蛋白胨提供氮源;氯化钠维持渗透压,琼脂是凝固剂。
用法:
        称取本品 40.0g,加热溶解于1000ml 蒸馏水中,121℃高压灭菌 15 分钟,冷至 45-50℃时,加入 5%-8% 无菌脱纤维羊血,混匀,倾入无菌平皿。 
 注意不要产生气泡。 
质量控制:

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