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碱性琼脂平板
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更新时间:2016-06-10  |  阅读:1519

详情介绍

碱性琼脂平板 Alkaline Agar 250g 用于分离霍乱弧菌。

用途:用于分离霍乱弧菌

碱性琼脂平板配方:(g/L)
蛋白胨
牛肉浸粉
氯化钠
琼脂
pH值8.4 ± 0.1      
10.0 
3.0 
5.0 
15.0
25℃
原理:
        蛋白胨、牛肉浸粉提供氮源、维生素和矿物质,氯化钠维持均衡的渗透压,琼脂是培养基的凝固剂。
用法:
         称取本品  33.0g,加热搅拌溶解于 1000ml 蒸馏水中,分装三角瓶,121℃高压灭
 菌 15 分钟,备用。

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