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碱性胆盐琼脂
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更新时间:2016-06-07  |  阅读:1644

详情介绍

碱性胆盐琼脂 Alkaline Bile Salt Agar 250g 用于分离霍乱弧菌。

用途:用于分离霍乱弧菌

碱性胆盐琼脂配方:(g/L)
蛋白胨
牛肉浸粉
氯化钠
牛胆盐
琼脂
PH值 8.3±8.5   
20.0
5.0
5.0
2.5 
15.0
25℃
原理:
        蛋白胨、牛肉浸粉提供碳氮源、维生素和生长因子;氯化钠维持均衡的渗透压;琼脂是培养基的凝固剂;牛胆盐抑制大肠菌群和其他杂菌的生长,有利于霍乱弧菌的生长。
用法:
        称取本品 47.5g,加热搅拌溶解于 1000ml蒸馏水中,分装三角瓶,121℃高压灭菌  15 分钟,
 备用。
质量控制:

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